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Image Search Results
Journal: bioRxiv
Article Title: B3GALT6 Promotes Dormant Breast Cancer Cell Survival and Recurrence by Enabling Heparan Sulfate-Mediated FGF Signaling
doi: 10.1101/2023.01.31.526529
Figure Lengend Snippet: A. Application of KEGG glycosaminoglycan (GAG) biosynthesis signatures for heparan sulfate/heparin and B. chondroitin sulfate/dermatan sulfate on MTB/TAN IVD temporal gene expression profiles. Circle=baseline, x=non-significant, empty triangles=trending significance 0.05< p <0.1, filled triangles= p <0.05, inverted triangles=decreased signature score, upright triangles=increased signature score. C. Heatmap of KEGG glycosaminoglycan (GAG) biosynthesis gene expression signatures for heparan sulfate/heparin (left) and D. chondroitin sulfate/dermatan sulfate (right) on MTB/TAN IVD temporal gene expression profiles. Black box represents the major group demonstrating dormancy-selective upregulation identified by hierarchical clustering. Red asterisks represent enzymes involved in tetrasaccharide linker synthesis during proteoglycan assembly. Blue boxes indicate enzymes involved in determining heparan sulfate synthesis, yellow boxes indicate enzymes involved in chondroitin sulfate synthesis. E. Immunofluorescence for heparan sulfate (left panels) or F. chondroitin sulfate (right panels) (red) in PTs derived from Her2-dependent-Cas9 cells with sg Rosa (green). Heparan sulfate/Chondroitin sulfate staining following heparin lyase or chondroitinase treatment is used as the negative control. Scale bar=100µm.
Article Snippet: For GAG immunofluorescence, specificity controls were included where tissue sections were treated with
Techniques: Gene Expression, Immunofluorescence, Derivative Assay, Staining, Negative Control